Application range of 3Cs technology expanded to orthogonal screens

Cetin R, Wegner M, Luwisch L, Saud S, Achmedov T, Süsser S, Vera-Guapi A, Müller K, Matthess Y, Quandt E, Schaubeck S, Beisel CL, Kaulich M. Optimized metrics for orthogonal combinatorial CRISPR screens. Sci Rep. 2023 13(1):7405.

 Open the paper: doi: 10.1038/s41598-023-34597-8

ReCo: a stand-alone and user-friendly analytics tool for analysis of PRCISR™ CRISPR screens

 Open the paper: doi: 10.1093/bioinformatics/btad448

3Cs multiplexing in action: a scalable method for combinatorial CRISPR screening

Diehl V, Wegner M, Grumati P, Husnjak K, Schaubeck S, Gubas A, Shah VJ, Polat IH, Langschied F, Prieto-Garcia C, Müller K, Kalousi A, Ebersberger I, Brandts CH, Dikic I, Kaulich M. Minimized combinatorial CRISPR screens identify genetic interactions in autophagy. Nucleic Acids Res 2021, 49(10): 5684-5704.

 Open the paper: doi: 10.1093/nar/gkab309

Take a deep tech dive into 3Cs PRCISR™ CRISPR gRNA library generation.

Wegner M, Husnjak K, Kaulich M. Unbiased and Tailored CRISPR/Cas gRNA Libraries by Synthesizing Covalently-closed-circular (3Cs) DNA. Bio Protoc 2020, 10(1): e3472.

 Open the paper: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842546/

First publication of 3Cs PRCISR™ CRISPR technology  – demonstrating  fidelity and performance of 3Cs reagents.

Wegner M, Diehl V, Bittl V, de Bruyn R, Wiechmann S, Matthess Y, Hebel M, Hayes MG, Schaubeck S, Benner C, Heinz S, Bremm A, Dikic I, Ernst A, Kaulich M. Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome. Elife 2019, 8: e42549.

 Open the paper: doi: 10.7554/eLife.42549

GIT Laborportal features 3Cs technology

Koch K, Kaulich M. 3Cs-Technologie zur Produktion von sgRNA-Bibliotheken. GIT Laborfachzeitschrift 2020, 1-2.

 Open the paper: https://analyticalscience.wiley.com/do/10.1002/gitfach.18708/full/