Single & combinatorial CRISPR library formats
PRCISR™ CRISPR libraries come in three sgRNA formats: single, fixed-pair, and multiplex.
Customize your plasmids with different promoters, tracrRNAs, antibiotic selection markers, fluorescence markers, barcodes, and single-cell RNA sequencing tags.
PRCISR™ CRISPR
Vivlion’s CRISPR-enabled Discovery Platform
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Single-targeting libraries |
Fixed-pair libraries |
Multiplex libraries |
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| 1 sgRNA per plasmid | Pre-defined pairs of sgRNAs per plasmid | Pairs of sgRNAs per plasmid according to set combinations | ||
| Single-targeting libraries deliver an individual sgRNA per cell. Our innovative approach decouples sequence diversity from distribution, allowing for comprehensive sequence diversity across all applications. | Fixed-pair libraries deliver pre-defined sgRNA pairs. For example, targeting the same gene for increasing editing efficiency or targeting fixed gene combinations of interest, or excising DNA. | Multiplex libraries provide all possible combinations of two sgRNA pools, allowing for exploration of combinatorial gene effects, e.g., functional buffering, synthetic vulnerabilities, and genetic interactions. | ||
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Why does library uniformity matter?
A genome-wide pooled CRISPR/Cas screen, with each gene targeted by 4 sgRNAs, contains more than 80.000 gRNAs. If these sgRNAs are not evenly distributed in the pool, then you will face the following challenges:
Low data quality: Poorly distributed sgRNAs will introduce bias into the gene editing events during the screen. This would lead to errors in the identification of enriched or depleted genes, which in turn severely impacts the reliability of the screening data. In the worst case, you will miss out on promising true targets while obtaining false positives.
Prohibitive experimental size: When sgRNAs are poorly distributed, to maintain statistical power, screening experiments may become excessively large to obtain valid results.
Vivlion’s PRCISR™ CRISPR technology enables uniformly distributed libraries by removing PCR and conventional cloning techniques during production. PRCISR™ CRISPR libraries are evenly distributed enabling minimized experimental setups and robust data quality.
Discover the editing efficiency of PRCISR™ CRISPR libraries
Vivlion’s unique technology for library production
CONVENTIONAL
methods for library preparation often suffer from
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PCR amplification bias, leading to uneven sgRNA distribution.
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Template switching issues, causing inaccurate library representation.
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Restriction and ligation artefacts, resulting in non-uniform libraries with high distribution skews.
VIVLION’S PRCISR™ CRISPR
technology produces superior library quality
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Uniformity of libraries: Our process results low skew libraries with uniform sgRNA distribution.
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No PCR bias: PCR amplification bias and template switching issues are avoided.
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No artefacts: common artefacts associated with conventional library preparation are eliminated.
Our featured PRCISR™ CRISPR libraries
Vivlion’s flagship genome-wide PRCISR™ CRISPR library, coming in single and fixed-pair formats.
Industry-leading uniformity, efficiency, and gene coverage.
Order now: See all Alexandria libraries on the products page
Pergamon: A minimized single-targeting genome-wide PRCISR™ CRISPR library.
Industry-leading uniformity, efficiency, and gene coverage.
Order now: See all Pergamon libraries on the products page
Industry-leading uniformity, efficiency, and coverage of gene family.
Order now: See all UPS libraries on the products page
Contact us
We would like to hear more about your CRISPR questions and applications – please reach out to us by completing the following form:
