PRCISR™ CRISPR
Vivlion’s CRISPR-enabled Discovery Platform
We offer support in the following areas
Custom library design: We will support you in the in silico design of sgRNA sequences, approve your client-provided sgRNA sequences (on-target scoring, off-target identification, detection of problematic sequences, identification/removal of duplicates), help you select control sequences, assist you with barcoding and single cell compatibility. All our PRCISR™ CRISPR libraries are quality controlled with NGS to ensure completeness and a uniform distribution.
Post-screen data analytics: We support you with sgRNA abundance and read counting, in silico quality control (enrichment and depletion of control sgRNAs; correlation on sgRNA, gene, and phenotype level), single edit enrichment and depletion analysis with established and proprietary workflows, multiplex enrichment and depletion analyses, genetic interaction mapping, function and pathway enrichment analyses, and visualization.
Optional: Data integration of prior knowledge and public resources (gene expression and function, protein-protein interaction, etc.).
Depending on you needs, we are open to discuss any additional requests for further analytical and visualization steps.
Basic service included in all Vivlion screens
Experimental design
- Empirically validated CRISPRko sgRNAs
- Approval of client-provided sgRNAs: on-target scoring, off-target identification, detection of undesired sequence patterns
- Selection of appropriate control sequences
- Barcoding
- Single cell RNA-seq compatibility
- Selection of appropriate screening parameters
NGS sample processing
- Sequencing strategies for single, fixed-pair, and multiplex
- Sample demultiplexing and read counting
- Sequencing depth
- Data deconvolution with ReCo
Screening sample processing
- Replicate correlation
- Effect size determination
- Performance of control sequences
Hit calling
- Enrichment/depletion analysis (e.g., viability, FACS reporter)
- Combinatorial gene effects (e.g., genetic interactions, synthetic lethality)
- Pipeline of established tools: MAGeCK, PinAPL-py, BAGEL, drugZ, Gemini, …
Further tailored services
Hit calling
- With customized pipelines
Data integration and interpretation
- Functional enrichment
- GO analysis
- Network biology
- And others
ReCo: automated NGS read-counting of single and combinatorial CRISPR gRNAs
ReCo was developed as a stand-alone and user-friendly analytics tool for generating read-count tables of single and combinatorial CRISPR library and screen-based NGS data.
Wegner M, Kaulich M. ReCo: automated NGS read-counting of single and combinatorial CRISPR gRNAs. Bioinformatics. 2023 Aug 1;39(8):btad448.
Contact us
We would like to hear more about your CRISPR questions and applications – please reach out to us by completing the following form:
